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Program Element                                                09/20/95 och

      Name of study:  Striped bass egg and larva survey
 
      Program manager
             Name:    Donald E. Stevens
             Agency:  California Department of Fish and Game
             Address: 4001 N. Wilson Way
                      Stockton CA 95205
 
             Phone:   209-948-7800
 
      Purpose/Objective:  To understand the factors that control the 
abundance and distribution of eggs and larva of striped bass in the 
estuary.  To estimate growth and mortality rates.
 
      Geographic range of field work:  Sampling sites are selected 
based on the expected flow patterns in the estuary.  Sites located 
in Carquinez Straits and western Suisun Bay are sampled in wet years 
but not in dry years.
 
       Number of sites:   The number of sites sampled in any one year 
is variable depending on conditions and the study plan for that year.  
Generally there are 32 sites or more sampled in the Delta and Suisun Bay.  
Upsteam sampling is done on the Sacramento River from Rio Vista to Colusa 
in some years to assess the temporal-spatial distribution of spawning.

 
       Period of record (Start year):  years sampled
       YEAR     SAMPLING
              FREQUENCY             STATIONS
       --------------------------------------------------------------------       
       1968   every 2nd day         1, 3 ,5, 7, 9, 11, 13, 15,17,19, 21,23, 
                                    25, 27, 29, 32, 33, 35, 37, 39, 41, 43, 
                                    45, 47, 49, 51, 53, 55, 57, 59, 61.
 
       1970          "              Suisun Bay/Delta sites as above plus 
                                    sporadic sampling of sites 63, 64, 65, 
                                    66.
 
       1971          "              Suisun Bay/Delta  sites as in 1968.
 
       1972          "              Estuarine sites plus Sacramento River 
                                    sites:  70, 71, 72, 73, 74, 75, 76, 77, 
                                    78, 79, 80, 81, 82, 83.
       1973          "                             "
       1975          "                             "
 
       1977          "              Suisun Bay/Delta sites and Sacramento
                                    River sites as in 1972 with the 
                                    addition of sporadic sampling of new 
                                    sites 735, 745, 755, 765, 775, 775, 
                                    785, 795, 805, 815, 825.
 
       1984   every 4th day         Sites as in 1972, plus sites 63-68,
              in Suisun Bay/        401,403, 407, 414, 416, 515.   
              L. Sacto River
              every second day
              only during the
              spawning season
              at other sites.
       1985           "             Sites: 5, 7, etc to 61; 63-68; 606-609; 
                                    515, 70-82; 735-825.
 
       1986           "             Sites: 1, 3, etc to 61; 63-68; 401,
                                    403, 407, 414, 416, 515, 70-83; 
                                    735-825.
 
       1988   every 4th day         Sites:  5, 7,  etc to 61;  63-68; 515, 
                                    606, 607, 609, 611, 906, 909.
              all sites            
 
       1989   1984 pattern          Sites:  5, 7, etc to 61;  65, 66, 
                                    70-75; 725-745; 906.
 
       1990   1984 pattern          Sites: 5, 7, etc to 61;  65-66;  906; 
                                    Upper Sacramento River only 78-82; 
                                    775-825.
 
       1991   1984 pattern          Sites: 5, 7, etc to 61;  65, 66, 70-82; 
                                    725-825; 906.
 
       1992   1984 pattern          Sites: 5, 7 etc to 61: 65-66; 70-80; 
                                    712-713, 715, 717, 735-795; new delta 
                                    smelt sites: 712-713, 715, 717; 903.
 
       1993   1984 pattern          Sites: 1, 3 etc to 61; 63-68, 414, 416, 
                                    515, 606, 607, 609, 906, 70-80; 
                                    725-795; 
                                    new delta smelt sites: 712-721,725;
                                    761, 903, 919-921, 923-926; 940,

       Size for complete data base for program element in KB (MB):
 
       Number of individual files:
               Six fish density files; 5 zooplankton files; 6 fish 
               abundance files.
 
       Sample frequency per time unit (second, week, month):
               Every other day or every fourth day as per above table.
 
       General category of data collected (e.g., media, flow, physical, 
       common chemicals, metals, pesticides, nutrients,  chlorophyll, 
       fish, invertebrates, or phyto/zooplankton):
              Fish(1968, 1970, 1971(late start), 1972, 1973(late start)
              1975, 1977(finished early due to boat breakdowns), 
              1984-1986, 1988-1993.
              Zooplankton at Suisun Bay /Delta stations only except in 
              1988 when we sampled the lower Sacramento River.  
              (1984-1986, 1988-1993), Chlorophyll(1984-1986,1988),  
              turbidity(1970-1972) secchi(since 1973), water 
              temperature(1975-present), specific conductance 
              (1970-1993; bottom EC starting in 1989).
 
       Comments about element (e.g., idiosyncracies, changes over time, 
       special events, etc.):
 
Field Sampling
       Gear type or field instrument used:
              A towed net mounted on skiis with a center mounted flow 
              meter.  The net frame and skiis weigh 41 lbs. Mouth  
              opening is variable from net to net but is in the range 
              of .400 to .440 in recent years.

       Brief description of sampling procedure, including sub-sampling, 
       sorting, identification of organisms (this should include 
       procedures for transport of sample from field to lab):
 
              Ichthyoplankton are sampled in diagonal tows usually of 
              10 minutes duration at each site with a 505 micron mesh 
              net.  The amount of cable warp used and the time at each 
              is determined by the depth of the site to assure that the
              net samples the water above the bottom.  Samples are   
              preserved in either 5 percent formalin or 95 percent  
              ethanol.  The ethanol is use to preserve otoliths for age 
              analysis of larvae.  A Clarke-Bumpus net of 154 micron 
              mesh is attached to the frame ofthe ichthyoplankton net 
              to take a simultaneous sample of the zooplankton to    
              measure the concentration of food available for fish.  If 
              there are blooms of the chain diatom,  Melosira occuring  
              the tows may be shortened to attempt to minimize net 
              clogging.

       Reference to any written protocols and how to obtain a copy:
              Protocols on current sampling practices are available on 
              request to the project manager.  A general detailed   
              description of the sampling is provided in Low and Miller,
              IESP Technical Report 11.
 
       Changes in gear or procedures which affected the data over time:
              A major change in the design and mesh size of the 
              ichthyoplankton net  was made in 1975.  The net used prior
              to 1975 was 900 micron mesh and the net was cone-shaped. 
              The new net design was a cylinder-cone net with 505 micron
              mesh openings.  Corrections to the catches in the years 
              before 1975 are described in Low and Miller,  IESP 
              Technical Report 11.

Laboratory analysis - Chemical
       Name and address of laboratory(s) running analysis:
 
              Current since (date):
              Historical lab (if known) or reference to other 
              documentation:
 
       Analytical methodology for each parameter or group of parameters:
 
       Reference to table which lists detection limits for each  
       parameter:
 
              Current procedure since (date):
              Historical procedures (if known) or reference to other 
              documentation:
 
       Quality assurance/control (QA/QC) protocols (if brief) or 
       one-line qualifier:
 
       Reference to any written QA/QC protocols and how to obtain a  
       copy:
 
Laboratory analysis - Biological
       Name and address of laboratory(s) running analysis:
              Biological samples are processed in  house.
              Current since (date): Since initiation of the program in 
              1966.
              Historical lab(if known) or reference to other 
              documentation:
 
       Methodology for each analysis:
              Samples are sorted in the laboratory. Fish eggs and larva 
              are  removed and identified in accordance with laboratory 
              protocols.
 
              Current procedure since (date):
              Historical procedures (if known) or reference to other 
              documentation:
 
       Reference used for identification of organisms:
              Mansueti, A. J.  and J. D. Hardy.  1967.  Development of 
              fishes of the Chesapeake Bay Region,  an atlas of egg, 
              larval and juvenile stages.  Part I. Natural Resources 
              Institute, University of Maryland. Port City Press. 
              Baltimore, Md. 202 p.
 
              Lippson, A. J. and R. L. Moran. 1974.  Manual for the 
              identification of early developmental stages of fishes of
              the Potomac River Estuary. Power Plant Siting Program of 
              the Maryland Department of Natural Resources. Baltimore, 
              Md. PPSP-MP-13. 282 p.
 
              Wang, Johnson C. S. 1981.  Taxonomy of the early life 
              stages of fishes-fishes of the Sacramento-Joaquin Estuary 
              and Moss Landing Harbor-Elkhorn Slough, California.  
              Ecological Analysts, Inc.,  Concord, California. 168 p.
 
              Wang, Johnson C. S. 1986.  Fishes of the Sacramento-San 
              Joaquin estuary and adjacent waters, California: A guide 
              to the early life histories.  Interagency Ecological Study
              Proran for the Sacramento-San Joaquin Estuary, Technical 
              Report No. 9.
 
       Location of reference collection (if one exists):
              Some reference material is located at the Bay-Delta 
              Division laboratory at:
              4001 N. Wilson Way
              Stockton CA 95205
 
       Reference to table listing scientific name, common name (if any),
       and code used:
              Codes will be defined in the documentation.